Mucoid Pseudomonas aeruginosa Can Produce Calcium-Gelled Biofilms Independent of the Matrix Components Psl and CdrA-Reference-Cited by-同舟云学术

Mucoid Pseudomonas aeruginosa Can Produce Calcium-Gelled Biofilms Independent of the Matrix Components Psl and CdrA

Published:2022-05-17 Issue:5 Volume:204 Page:
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Jacobs Holly M.¹²,O’Neal Lindsey²,Lopatto Edward²,Wozniak Daniel J.³⁴,Bjarnsholt Thomas⁵⁶^ORCID,Parsek Matthew R.²

Affiliation:

1. Molecular and Cellular Biology Graduate Program, University of Washington, Seattle, Washington, USA

2. Department of Microbiology, University of Washington, Seattle, Washington, USA

3. Department of Microbial Infection and Immunity, The Ohio State University, Columbus, Ohio, USA

4. Department of Microbiology, The Ohio State University, Columbus, Ohio, USA

5. Costerton Biofilm Center, Department of Immunology and Microbiology, Faculty of Health and Medical Science, University of Copenhagen, Copenhagen, Denmark

6. Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark

Abstract

The opportunistic pathogen Pseudomonas aeruginosa produces a complex biofilm matrix comprised of exopolysaccharides (EPSs), nucleic acids, and proteins. P. aeruginosa biofilm formation canonically depends on a variable combination of the exopolysaccharides Psl and Pel and the matrix protein CdrA. We demonstrate that mucoid P. aeruginosa , which overproduces the EPS alginate, possesses an entirely alternate and calcium-dependent method of biofilm formation.

Funder

American Heart Association

Cystic Fibrosis Foundation

HHS | National Institutes of Health

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.00568-21

Reference57 articles.

1. Cystic fibrosis;Asay LD;Calif Med,1965