Affiliation:
1. MICOER Institute, Munich, Germany
2. Johnson & Johnson Pharmaceutical Research and Development, Raritan, New Jersey
Abstract
ABSTRACT
Pandoraea
spp. are gram-negative, glucose nonfermenting rods detectable in blood cultures and sputa of cystic fibrosis patients. They are resistant to various antibiotic groups, with imipenem being the only active β-lactam. We isolated an imipenem-resistant (MIC, 64 μg/ml)
Pandoraea pnomenusa
strain from a cystic fibrosis patient. Cloning and sequencing identified two β-lactamases of Bush group 2d, namely, the known OXA-33, located on an integron, and the novel carbapenem-hydrolyzing oxacillinase OXA-62. OXA-62 is only distantly related to other oxacillinases (OXA-50 being closest with 43% amino acid identity). It hydrolyzes penicillins, oxacillin, imipenem, and meropenem but not expanded-spectrum cephalosporins. The
bla
OXA-62
gene is chromosome located. No transposable elements were found in its genetic neighborhood. With OXA-62-specific primers,
bla
OXA-62
could be identified in all
P. pnomenusa
strains and appears to be species specific. This additional mechanism of carbapenem resistance further complicates the treatment of infections caused by
P. pnomenusa
.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
Cited by
39 articles.
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