TaqMan Probe-Based Real-Time PCR Assay for Detection and Discrimination of Class I, II, and III tfdA Genes in Soils Treated with Phenoxy Acid Herbicides-Reference-Cited by-同舟云学术

TaqMan Probe-Based Real-Time PCR Assay for Detection and Discrimination of Class I, II, and III tfdA Genes in Soils Treated with Phenoxy Acid Herbicides

Published:2009-05 Issue:9 Volume:75 Page:2969-2972
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Bælum Jacob¹,Jacobsen Carsten S.¹²

Affiliation:

1. Geological Survey of Denmark and Greenland (GEUS), Department of Geochemistry, Øster Voldgade 10, DK-1350 Copenhagen K, Denmark

2. Faculty of Life Sciences, Department of Natural Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark

Abstract

ABSTRACT Separate quantification of three classes of tfdA genes was performed using TaqMan quantitative real-time PCR for 13 different soils subsequent to mineralization of three phenoxy acids. Class III tfdA genes were found to be involved in mineralization more often than class I and II tfdA genes.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.02051-08

Reference19 articles.

1. Degradation of 4-Chloro-2-Methylphenoxyacetic Acid in Top- and Subsoil Is Quantitatively Linked to the Class III tfdA Gene

2. Bælum, J., M. H. Nicolaisen, W. E. Holben, B. W. Strobel, J. Sorensen, and C. S. Jacobsen. 2008. Direct analysis of tfdA gene expression by indigenous bacteria in phenoxy acid amended agricultural soil. ISME J.2:677-687.

3. Metabolism of 4-Chloro-2-Methylphenoxyacetic Acid by Soil Bacteria

4. Demontis, M. A., S. O. Cacciola, M. Orru, V. Balmas, V. Chessa, B. E. Maserti, L. Mascia, F. Raudino, G. M. D. S. Quirico, and Q. Migheli. 2008. Development of real-time PCR systems based on SYBR Green I and TaqMan technologies for specific quantitative detection of Phoma tracheiphila in infected Citrus. Eur. J. Plant Pathol.120:339-351.

5. Transposon mutagenesis and cloning analysis of the pathways for degradation of 2,4-dichlorophenoxyacetic acid and 3-chlorobenzoate in Alcaligenes eutrophus JMP134(pJP4)

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