Epitope-Blocking Enzyme-Linked Immunosorbent Assays for Detection of West Nile Virus Antibodies in Domestic Mammals

Author:

Blitvich Bradley J.1,Bowen Richard A.2,Marlenee Nicole L.1,Hall Roy A.3,Bunning Michel L.45,Beaty Barry J.1

Affiliation:

1. Arthropod-Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology

2. Animal Reproduction and Biotechnology Laboratory, Equine Center, Colorado State University, Fort Collins, Colorado 80523

3. Department of Microbiology and Parasitology, The University of Queensland, St. Lucia, Queensland, Australia 4072

4. Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522

5. Office of the Surgeon General, United States Air Force, Washington, D.C. 20332

Abstract

ABSTRACT We evaluated the ability of epitope-blocking enzyme-linked immunosorbent assays (ELISAs) to detect West Nile virus (WNV) antibodies in domestic mammals. Sera were collected from experimentally infected horses, cats, and pigs at regular intervals and screened in ELISAs and plaque reduction neutralization tests. The diagnostic efficacies of these techniques were similar.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference17 articles.

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2. Epitope-Blocking Enzyme-Linked Immunosorbent Assays for the Detection of Serum Antibodies to West Nile Virus in Multiple Avian Species

3. Burke D. S. and T. P. Monath. 2001. Flaviviruses p. 1043-1126. In D. M. Knipe and P. M. Howley (ed.) Fields virology 4th ed. Lippincott Williams and Wilkins Philadelphia Pa.

4. Casals J. 1973. Arbovirus infections p. 99-117. In J. R. Paul and C. White (ed.) Serological epidemiology. Academic Press New York N.Y.

5. West Nile Virus Recombinant DNA Vaccine Protects Mouse and Horse from Virus Challenge and Expresses In Vitro a Noninfectious Recombinant Antigen That Can Be Used in Enzyme-Linked Immunosorbent Assays

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