Evaluation of the Epidemiologic Utility of Secondary Typing Methods for Differentiation of Mycobacterium tuberculosis Isolates

Author:

Kwara Awewura1,Schiro Ronald2,Cowan Lauren S.3,Hyslop Newton E.1,Wiser Mark F.4,Roahen Harrison Stephanie5,Kissinger Patricia5,Diem Lois3,Crawford Jack T.3

Affiliation:

1. Section of Adult Infectious Diseases, Tulane University Health Sciences Center

2. Office of Public Health, Louisiana Department of Health and Hospitals, New Orleans, Louisiana 70112

3. Division of AIDS, STD, and TB Laboratory Research, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333

4. Department of Tropical Medicine

5. Department of Epidemiology, Tulane University School of Public HealthTropical Medicine

Abstract

ABSTRACT Spoligotyping and mycobacterial interspersed repetitive unit-variable-number tandem repeat analysis (MIRU-VNTR) were evaluated for the ability to differentiate 64 Mycobacterium tuberculosis isolates from 10 IS 6110 -defined clusters. MIRU-VNTR performed slightly better than spoligotyping in reducing the number of clustered isolates and the sizes of the clusters. All epidemiologically related isolates remained clustered by MIRU-VNTR but not by spoligotyping.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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