Epidemiologic Usefulness of Spoligotyping for Secondary Typing of Mycobacterium tuberculosis Isolates with Low Copy Numbers of IS 6110-Reference-Cited by-同舟云学术

Epidemiologic Usefulness of Spoligotyping for Secondary Typing of Mycobacterium tuberculosis Isolates with Low Copy Numbers of IS 6110

Published:2001-10 Issue:10 Volume:39 Page:3709-3711
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Cronin Wendy A.¹,Golub Jonathan E.¹,Magder Laurence S.²,Baruch Nancy G.¹,Lathan Monica J.¹,Mukasa Leonard N.¹,Hooper Nancy³,Razeq Jafar H.³,Mulcahy Donna⁴,Benjamin William H.⁵,Bishai William R.⁶

Affiliation:

1. Division of TB Control, Refugee and Migrant Health,1 and

2. Maryland Department of Health and Mental Hygiene, and Department of Epidemiology and Preventive Medicine, University of Maryland—Baltimore,2 Baltimore, Maryland 21201;

3. Public Health Microbiology, Laboratories Administration,3

4. Respiratory Disease Division, Department of Public Health, Bureau of Clinical Laboratories, Montgomery, Alabama 36130-30174; and

5. Department of Pathology, University of Alabama, Birmingham, Alabama 352945

6. Department of International Health, School of Public Health and Hygiene, Johns Hopkins University, Baltimore, Maryland 212056;

Abstract

ABSTRACT Restriction fragment length polymorphism (RFLP) analysis of IS 6110 is commonly used to DNA fingerprint Mycobacterium tuberculosis . However, low-copy (≤5) IS 6110 M. tuberculosis strains are poorly differentiated, requiring secondary typing. When spoligotyping was used as the secondary method, only 13% of Maryland culture-positive tuberculosis (TB) patients with low-copy IS 6110 -spoligotyped clustered strains had epidemiologic linkages to another patient, compared to 48% of those with high-copy strains clustered by IS 6110 alone ( P < 0.01). Spoligotyping did not improve a population-based molecular epidemiologic study of recent TB transmission.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.39.10.3709-3711.2001

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