Multiplex PCR Assay for Identification of Human Diarrheagenic Escherichia coli-Reference-Cited by-同舟云学术

Multiplex PCR Assay for Identification of Human Diarrheagenic Escherichia coli

Published:2003-06 Issue:6 Volume:41 Page:2669-2671
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Toma Claudia¹,Lu Yan¹,Higa Naomi¹,Nakasone Noboru¹,Chinen Isabel²,Baschkier Ariela²,Rivas Marta²,Iwanaga Masaaki¹

Affiliation:

1. Department of Bacteriology, Faculty of Medicine, University of the Ryukyus, Nishihara, Okinawa 903-0215, Japan

2. Servicio Fisiopatogenia, Instituto Nacional de Enfermedades Infecciosas, ANLIS-“Dr. Carlos G. Malbrán,” 1281 Buenos Aires, Argentina

Abstract

ABSTRACT A multiplex PCR assay for the identification of human diarrheagenic Escherichia coli was developed. The targets selected for each category were eae for enteropathogenic E . coli , stx for Shiga toxin-producing E . coli , elt and est for enterotoxigenic E . coli , ipaH for enteroinvasive E . coli , and aggR for enteroaggregative E . coli . This assay allowed the categorization of a diarrheagenic E . coli strain in a single reaction tube.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.41.6.2669-2671.2003

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