Affiliation:
1. Squibb Institute for Medical Research, New Brunswick, New Jersey 08903
Abstract
A method has been devised for the determination of 9α-fluorohydrocortisone and 9α-fluoro-16α-hydroxyhydrocortisone in fermentation broths. The method involves extraction of the two steroids from the broth with ethyl acetate, separation through the formation of the water-soluble borate complex of the vicinally hydroxylated steroid, and estimation of each steroid spectrophotometrically.
Publisher
American Society for Microbiology
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine