Aflatoxin-Producing Strains of Aspergillus flavus Detected by Fluorescence of Agar Medium Under Ultraviolet Light-Reference-Cited by-同舟云学术

Aflatoxin-Producing Strains of Aspergillus flavus Detected by Fluorescence of Agar Medium Under Ultraviolet Light

Published:1974-06 Issue:6 Volume:27 Page:1118-1123
ISSN:0003-6919
Container-title:Applied Microbiology
language:en
Short-container-title:Appl Microbiol

Author:

Hara S.¹,Fennell D. I.¹,Hesseltine C. W.¹

Affiliation:

1. Northern Regional Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Peoria, Illinois 61604

Abstract

The fluorescence method of detecting aflatoxin-producing strains of Aspergillus flavus and related species utilizes the ultraviolet-induced fluorescence of aflatoxin produced in a modified Czapek's solution agar containing corn steep liquor, HgCl 2 , and (NH 4 )H 2 PO 4 instead of NaNO 3 . The presence of aflatoxin is confirmed by thin-layer chromatography of CHCl 3 extracts of the fluorescing agar.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Link

https://journals.asm.org/doi/pdf/10.1128/am.27.6.1118-1123.1974

Reference10 articles.

1. Mycotoxins. 1. Studies on aflatoxin derived from contaminated peanut meal and certain strains of Aspergillus flavus;Armbrecht B. H.;J. Ass. Off. Agr. Chem.,1963

2. Association of Official Analytical Chemists. 1970. Official methods of analysis p. 436 11th ed. sect. 26.

3. Production of aflatoxin by the culture of strains of Aspergillus flavus-oryzae on sterilized peanuts;Codner R. C.;Biotechnol. Bioeng.,1963

4. Aflatoxin formation by Aspergillus flavus;Hesseltine C. W.;Bacteriol. Rev.,1966

5. Studies on the fluorescent substances produced by Aspergillus fungi. VIII. Purification and isolation of asperopterin B and chemical properties of asperopterin B and A;Kaneko Y.;J. Ferment. Technol.,1969

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