Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Direct Bacterial Identification from Positive Blood Culture Pellets-Reference-Cited by-同舟云学术

Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Direct Bacterial Identification from Positive Blood Culture Pellets

Published:2010-04 Issue:4 Volume:48 Page:1481-1483
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Prod'hom Guy¹,Bizzini Alain¹,Durussel Christian¹,Bille Jacques¹,Greub Gilbert¹

Affiliation:

1. Institute of Microbiology, University of Lausanne and University Hospital Center, Lausanne, Switzerland

Abstract

ABSTRACT An ammonium chloride erythrocyte-lysing procedure was used to prepare a bacterial pellet from positive blood cultures for direct matrix-assisted laser desorption-ionization time of flight (MALDI-TOF) mass spectrometry analysis. Identification was obtained for 78.7% of the pellets tested. Moreover, 99% of the MALDI-TOF identifications were congruent at the species level when considering valid scores. This fast and accurate method is promising.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01780-09

Reference7 articles.

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