Novel Observation of Hot-Cold-Hot Hemolysis Exhibited by Group B Streptococci

Author:

Okazaki Norio1,Osawa Ro2,Suzuki Rieko1,Nikkawa Takayasu1,Whiley Robert A.3

Affiliation:

1. Department of Bacteriology and Pathology, Kanagawa Prefectural Public Health Laboratory, Nakao 1-1-1, Asahi-ku, Yokohama 241-0815

2. Department of Bioscience, Graduate School of Science and Technology, Kobe University, Rokko-dai 1-1, Nada-ku, Kobe 657-8501, Japan

3. Department of Oral Microbiology, St. Bartholomew's and Royal London School of Medicine and Dentistry, London E1 2AD, United Kingdom

Abstract

ABSTRACT Six human isolates of group B streptococci (GBS) were cultured on blood agar anaerobically at 37°C for 18 h and then at 4°C for 6 h and reincubated anaerobically at 37°C for 6 h. Three of the strains showed a marked enlargement of the hemolysis zone compared with that obtained after hot-only (37°C for 18 h) or hot-cold (37°C for 18 h and then 4°C for 6 h) treatment. Subsequent broth culture experiments revealed that enhanced hemolytic activity due to hot-cold-hot treatment was observed in all 6 GBS strains when cultured in the presence of starch.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Streptococcus;Bergey's Manual of Systematics of Archaea and Bacteria;2015-09-14

2. Bacterial virulence factors in neonatal sepsis: group B streptococcus;Current Opinion in Infectious Diseases;2004-06

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