Extended-Spectrum Properties of CMY-30, a Val211Gly Mutant of CMY-2 Cephalosporinase-Reference-Cited by-同舟云学术

Extended-Spectrum Properties of CMY-30, a Val211Gly Mutant of CMY-2 Cephalosporinase

Published:2009-08 Issue:8 Volume:53 Page:3520-3523
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Kotsakis Stathis D.¹,Papagiannitsis Costas C.¹,Tzelepi Eva¹,Tzouvelekis Leonidas S.²,Miriagou Vivi¹

Affiliation:

1. Laboratory of Bacteriology, Hellenic Pasteur Institute

2. Department of Microbiology, School of Medicine, University of Athens, Greece

Abstract

ABSTRACT CMY-30, a Val211Gly mutant of CMY-2 cephalosporinase, was derived by mutagenesis. The hydrolytic efficiency of CMY-30 against expanded-spectrum cephalosporins was higher than that of CMY-2 due to increased k cat values. Findings indicate a role of the Ω loop residue 211 in determining the substrate specificities of CMYs also corroborated by modeling studies.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.00219-09

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