Defining Stable Reference Genes in HIV Latency Reversal Experiments-Reference-Cited by-同舟云学术

Defining Stable Reference Genes in HIV Latency Reversal Experiments

Published:2021-05-10 Issue:11 Volume:95 Page:
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Ceriani Cristina¹²,Streeter Gabrielle S.³,Lemu Kena J.¹,James Katherine S.¹²,Ghofrani Simon¹²,Allard Brigitte¹²,Shook-Sa Bonnie E.³,Margolis David M.¹²⁴,Archin Nancie M.¹²^ORCID

Affiliation:

1. UNC HIV Cure Center, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA

2. Department of Medicine, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA

3. Biostatistics Core, Center for AIDS Research, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA

4. Department of Microbiology and Immunology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA

Abstract

Latency-reversing agents (LRAs) are ubiquitously used in the “shock-and-kill” HIV cure strategy, and their performance is often evaluated by ex vivo quantification of cell-associated HIV RNA. HIV RNA, measured by quantitative PCR (qPCR), is often normalized to internal reference genes, but the expression of these genes should not be influenced by the experimental settings.

Funder

HHS | National Institutes of Health

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.02305-20

Reference44 articles.

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