The Helicobacter pylori CrdRS Two-Component Regulation System (HP1364/HP1365) Is Required for Copper-Mediated Induction of the Copper Resistance Determinant CrdA-Reference-Cited by-同舟云学术

The Helicobacter pylori CrdRS Two-Component Regulation System (HP1364/HP1365) Is Required for Copper-Mediated Induction of the Copper Resistance Determinant CrdA

Published:2005-07 Issue:13 Volume:187 Page:4683-4688
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Waidner Barbara¹,Melchers Klaus²³,Stähler Frank Nils¹,Kist Manfred¹,Bereswill Stefan¹

Affiliation:

1. Department of Medical Microbiology and Hygiene, Institute of Medical Microbiology and Hygiene, University Hospital Freiburg, Hermann-Herder-Straße 11, D-79104 Freiburg, Germany

2. ALTANA Research Institute, 610 Lincoln Street, Waltham, Massachusetts 02451

3. ALTANA Pharma AG, Department of Bioinformatics, Byk-Gulden-Str. 2, D-78467 Konstanz, Germany

Abstract

ABSTRACT Here we describe that the Helicobacter pylori sensor kinase produced by HP1364 and the response regulator produced by HP1365 and designated CrdS and CrdR, respectively, are both required for transcriptional induction of the H. pylori copper resistance determinant CrdA by copper ions. CrdRS-deficient mutants lacked copper induction of crdA expression and were copper sensitive. A direct role of CrdR in transcriptional regulation of crdA was confirmed by in vitro binding of CrdR to the crdA upstream region. A 21-nucleotide sequence located near the crdA promoter was shown to be required for CrdR binding.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.187.13.4683-4688.2005

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