Affiliation:
1. Department of Microbiology and Immunology, Louisiana State University Health Sciences Center, Shreveport, Louisiana 71130-3932
Abstract
ABSTRACT
A second mutation has recently been identified in the previously described
Brucella abortus htrA
mutant PHE1. As a result of this finding, a new
B. abortus htrA
mutant, designated RWP11, was constructed to evaluate the biological function of the
Brucella
HtrA protease. RWP11 is more sensitive to oxidative killing in vitro and less resistant to killing by cultured murine neutrophils and macrophages than the virulent parental strain 2308 but is not attenuated in BALB/c mice through 4 weeks postinfection. The in vitro phenotype of
B. abortus
RWP11 is consistent with the proposed function of bacterial HtrA proteases as components of a secondary line of defense against oxidative damage. The in vivo phenotype of this mutant, however, indicates that, unlike the corresponding
Salmonella
and
Yersinia
proteins,
Brucella
HtrA does not play a critical role in virulence in the mouse model.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Cited by
30 articles.
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