Role of Polyphosphate Kinase in Biofilm Formation by Porphyromonas gingivalis-Reference-Cited by-同舟云学术

Role of Polyphosphate Kinase in Biofilm Formation by Porphyromonas gingivalis

Published:2002-08 Issue:8 Volume:70 Page:4708-4715
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Chen Wen¹,Palmer Robert J.²,Kuramitsu Howard K.¹

Affiliation:

1. Department of Oral Biology, State University of New York, Buffalo, New York 14214

2. Oral Infection and Immunity Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland 20892

Abstract

ABSTRACT In order to assess the role of polyphosphate kinase (PPK) in the physiology of Porphyromonas gingivalis , a ppk gene mutant, CW120, was constructed and characterized. P. gingivalis was demonstrated to synthesize short-chain polyphosphate (polyP) but not long-chain polyP. CW120 failed to survive in the stationary phase as well as the parental cell did, and it was attenuated in biofilm formation on polyvinylchloride and glass surfaces. Furthermore, the complementation by insertion of an intact copy of the ppk gene into the mutant CW120 restored its biofilm formation and stationary-phase survival. These results suggest that PPK may be important for incorporation of these organisms into subgingival plaque in the human oral cavity.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/IAI.70.8.4708-4715.2002

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