Functional analysis of polyphosphate in Myxococcus xanthus

Author:

Harita Daiki1,Matsukawa Hiroka1,Kimura Yoshio1

Affiliation:

1. Kagawa University

Abstract

Abstract Myxococcus xanthus synthesizes polyphosphates (polyPs) with polyphosphate kinase 1 (Ppk1) and degrades short- and long-chain polyPs with the exopolyphosphatases, Ppx1 and Ppx2, respectively. M. xanthus also expresses polyP:AMP phosphotransferase (Pap) which generates ADP from polyPs and AMP. M. xanthus synthesized polyPs during the stationary phase; the ppk1 mutant showed no difference in growth during the exponential growth phase but died earlier than the wild-type and ppx2 mutant after the stationary phase. In addition, M. xanthus cells cultured in phosphate-starved medium, 0.07 mM H2O2-supplemented medium, or nutrient-deficient medium (CF medium) increased the intracellular polyP levels by six- to eight-fold after 6 h of incubation. However, the growth of ppk1 and ppx2 mutants in phosphate-starved medium and 0.07 mM H2O2 supplemented medium was not significantly different from that of wild-type strains, nor was there a significant difference in fruiting body formation and sporulation on CF medium. The adenylate energy charge (AEC) values of the wild-type strain and the ppk1, ppx2, and pap mutant strains at the exponential growth phase were approximately 0.80. The AEC of the wild-type strain did not change until day 3 of development, whereas the AEC values of the ppk1 and pap mutant strains decreased to 0.77 due to an increase in AMP and a decrease in ADP. Spores of ppk1 and pap mutants in the nutrient medium germinated later than those of the wild-type strain. These results suggested that polyPs produced during development may play an important role in cellular energy homeostasis by being used to convert AMP to ADP via Pap.

Publisher

Research Square Platform LLC

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3