Detection of erythromycin-resistant determinants by PCR-Reference-Cited by-同舟云学术

Detection of erythromycin-resistant determinants by PCR

Published:1996-11 Issue:11 Volume:40 Page:2562-2566
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Sutcliffe J¹,Grebe T¹,Tait-Kamradt A¹,Wondrack L¹

Affiliation:

1. Department of Infectious Diseases, Pfizer, Inc., Groton, Connecticut 06340, USA. Joyce_A_Sutcliffe@groton.pfizer.com

Abstract

Erythromycin resistance determinants include Erm methylases, efflux pumps, and inactivating enzymes. To distinguish the different mechanisms of resistance in clinical isolates, PCR primers were designed so that amplification of the partial gene products could be detected in multiplex PCRs. This methodology enables the direct sequencing of amplified PCR products that can be used to compare resistance determinants in clinical strains. Further, this methodology could be useful in surveillance studies of erythromycin-resistant determinants.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.40.11.2562

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