Detection of erythromycin resistance by the polymerase chain reaction using primers in conserved regions of erm rRNA methylase genes-Reference-Cited by-同舟云学术

Detection of erythromycin resistance by the polymerase chain reaction using primers in conserved regions of erm rRNA methylase genes

Published:1990-10 Issue:10 Volume:34 Page:2024-2026
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Arthur M¹,Molinas C¹,Mabilat C¹,Courvalin P¹

Affiliation:

1. Unité des Agents Antibactériens, Institut Pasteur, Paris, France.

Abstract

Genes belonging to different erm DNA hybridization classes were selectively amplified by polymerase chain reaction with a pair of oligonucleotides that corresponded to conserved amino acid motifs in known ERM methylases. Identification of the resistance mechanism was possible despite substantial nucleotide sequence diversity among the erythromycin resistance genes.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.34.10.2024

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