Genome-Wide CRISPR Screen Identifies RACK1 as a Critical Host Factor for Flavivirus Replication-Reference-Cited by-同舟云学术

Genome-Wide CRISPR Screen Identifies RACK1 as a Critical Host Factor for Flavivirus Replication

Published:2021-11-23 Issue:24 Volume:95 Page:
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Shue Byron¹,Chiramel Abhilash I.²,Cerikan Berati³,To Thu-Hien⁴,Frölich Sonja¹,Pederson Stephen M.⁴,Kirby Emily N.¹,Eyre Nicholas S.¹,Bartenschlager Ralf F. W.³^ORCID,Best Sonja M.²,Beard Michael R.¹^ORCID

Affiliation:

1. Research Centre for Infectious Diseases, Department of Molecular and Biomedical Science, The University of Adelaide, Adelaide, Australia

2. Rocky Mountain Laboratories, National Institutes of Health (NIH), Hamilton, Montana, USA

3. Department of Infectious Diseases, Molecular Virology, Heidelberg University, Heidelberg, 69120 Germany

4. Bioinformatics Hub, The University of Adelaide, Adelaide, Australia

Abstract

Cellular factors are critical in all facets of viral lifecycles, where overlapping interactions between the virus and host can be exploited as possible avenues for the development of antiviral therapeutics. Using a genome-wide CRISPR knockout screening approach to identify novel cellular factors important for flavivirus replication we identified RACK1 as a pro-viral host factor for both mosquito- and tick-borne flaviviruses in addition to SARS-CoV-2.

Funder

Department of Health | National Health and Medical Research Council

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.00596-21

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