Comparative Study Using Amplified Fragment Length Polymorphism Fingerprinting, PCR Genotyping, and Phenotyping To Differentiate Campylobacter fetus Strains Isolated from Animals

Author:

Wagenaar Jaap A.1,van Bergen Marcel A. P.1,Newell Diane G.2,Grogono-Thomas Rose2,Duim Birgitta1

Affiliation:

1. Institute for Animal Science and Health, Lelystad, The Netherlands,1 and

2. Veterinary Laboratories Agency (Weybridge), New Haw, United Kingdom2

Abstract

ABSTRACT A collection of Campylobacter fetus strains, including both C. fetus subsp. fetus and C. fetus subsp. venerealis , were phenotypically identified to the subspecies level and genotypically typed by PCR and amplified fragment length polymorphism (AFLP) analysis. Phenotypic subspecies determination methods were unreliable. Genotyping of the strains by PCR and AFLP showed a clear discrimination between the two subspecies.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference18 articles.

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2. Transduction and mutation to glycine tolerance in Vibrio fetus;Chang W.;Am. J. Vet. Res.,1971

3. Computer-Assisted Analysis and Epidemiological Value of Genotyping Methods for Campylobacter jejuni and Campylobacter coli

4. DNA fingerprinting of Campylobacter fetus using cloned constructs of ribosomal RNA and surface array protein genes;Denes A. S.;Vet. Microbiol.,1997

5. High-Resolution Genotyping of Campylobacter Strains Isolated from Poultry and Humans with Amplified Fragment Length Polymorphism Fingerprinting

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