Abstract
AbstractCampylobacter fetus comprises two closely related mammal-associated subspecies: Campylobacter fetus subsp. fetus (Cff) and Campylobacter fetus subsp. venerealis (Cfv). The latter causes bovine genital campylobacteriosis, a sexually-transmitted disease endemic in Spain that results in significant economic losses in the cattle industry. Here, 33 C. fetus Spanish isolates were whole-genome sequenced and compared with 62 publicly available C. fetus genomes from other countries. Genome-based taxonomic identification revealed high concordance with in silico PCR, confirming Spanish isolates as Cff (n = 4), Cfv (n = 9) and Cfv biovar intermedius (Cfvi, n = 20). MLST analysis assigned the Spanish isolates to 6 STs, including three novel: ST-76 and ST-77 for Cfv and ST-78 for Cff. Core genome SNP phylogenetic analysis of the 95 genomes identified multiple clusters, revealing associations at subspecies and biovar level between genomes with the same ST and separating the Cfvi genomes from Spain and other countries. A genome-wide association study identified pqqL as a Cfv-specific gene and a potential candidate for more accurate identification methods. Functionality analysis revealed variations in the accessory genome of C. fetus subspecies and biovars that deserve further studies. These results provide valuable information about the regional variants of C. fetus present in Spain and the genetic diversity and predicted functionality of the different subspecies.
Funder
Ministerio de Ciencia e Innovación
Ekonomiaren Garapen eta Lehiakortasun Saila, Eusko Jaurlaritza
Publisher
Springer Science and Business Media LLC
Reference60 articles.
1. van Bergen, M., van Putten, J. P. M., Dingle, K. E., Blaser, M. J. & Wagenaar, J. A. Isolation, identification, subspecies differentiation, and typing of Campylobacter fetus. In Campylobacter (eds Nachamkin, I. et al.) 213–225 (ASM Press, 2008).
2. Fitzgerald, C. et al. Campylobacter fetus subsp. testudinum subsp. nov., isolated from humans and reptiles. Int. J. Syst. Evol. Microbiol. 64, 2944–2948 (2014).
3. Gilbert, M. J. et al. Comparative genomics of Campylobacter fetus from reptiles and mammals reveals divergent evolution in host-associated lineages. Genome Biol. Evol. 8, 2006–2019 (2016).
4. Sprenger, H., Zechner, E. L. & Gorkiewicz, G. So close and yet so far—Molecular microbiology of Campylobacter fetus subspecies. Eur. J. Microbiol. Immunol. 2, 66–75 (2012).
5. Nadin-Davis, S. A. et al. A comparison of fourteen fully characterized mammalian-associated Campylobacter fetus isolates suggests that loss of defense mechanisms contribute to high genomic plasticity and subspecies evolution. PeerJ 9, e10586 (2021).