Affiliation:
1. Department of Medical Microbiology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada
2. National Microbiology Laboratory, Canadian Science Centre for Human and Animal Health, 1015 Arlington Street, Winnipeg, MB R3E 3R2,
Canada
Abstract
ABSTRACT
Aurintricarboxylic
acid (ATA) has been shown to inhibit the replication of viruses from
several different families, including human immunodeficiency virus,
vesicular stomatitis virus, and the coronavirus causing severe acute
respiratory syndrome. This study characterizes the inhibitory effect of
ATA on vaccinia virus replication in HeLa, Huh7, and AD293 cells.
Vaccinia virus replication is significantly abrogated upon ATA
treatment, which is associated with the inhibition of early viral gene
transcription. This inhibitory effect may be attributed to two
findings. First, ATA blocks the phosphorylation of extracellular
signal-regulated kinase 1/2, an event shown to be essential for
vaccinia virus replication. Second, ATA inhibits the phosphatase
activity of the viral enzyme H1L, which is required to initiate viral
transcription. Thus, ATA inhibits vaccinia virus replication by
targeting both cellular and viral factors essential for the early stage
of
replication.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
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