Affiliation:
1. Complex Carbohydrate Research Center, University of Georgia, Athens 30602-4712.
Abstract
The polysaccharide components from cultured cells of Rhizobium fredii USDA205 and Rhizobium meliloti AK631 were extracted with hot phenol-water and separated by repetitive gel filtration chromatography. Polyacrylamide gel electrophoresis, nuclear magnetic resonance spectrometry, and gas chromatography analyses showed that both of these bacterial species produce unique polysaccharides that contain a high proportion of 3-deoxy-D-manno-2-octulosonic acid (Kdo). These polysaccharides, which constituted a major portion of the extracted carbohydrate, are not excreted into the growth media (i.e., they are not extracellular polysaccharides) and are structurally distinct from the lipopolysaccharides. The primary structure of the preponderant polysaccharide from R. fredii USDA205 was determined by high-performance anion-exchange liquid chromatography, nuclear magnetic resonance spectrometry, fast atom bombardment-mass spectrometry, and gas chromatography-mass spectrometry; it consists of repeating units of [-->3)-alpha-D-Galp-(1-->5)-beta-D-Kdop-(2-->]n. This molecule is structurally analogous to the constituents of one subgroup of K antigens (capsular polysaccharides) produced by Escherichia coli. Polysaccharides of this type have not previously been identified as components of rhizobial cells. The Kdo-containing polysaccharide from R. meliloti, which has not been completely characterized, appears to be structurally related to that of R. fredii.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference44 articles.
1. Nuclear magnetic resonance spectroscopy in the study of mono- and oligosaccharides. Annu;Bock K.;Rep. NMR Spectrosc.,1982
2. A mutation that blocks exopolysaccharide synthesis prevents nodulation of peas by Rhizobium leguminosarum but not beans by R. phaseoli and is corrected by cloned DNA from Rhizobium or the phytopathogen;Borthakur D.;Xanthomonas. Mol. Gen. Genet.,1986
3. Common loci for Agrobacterium tumefaciens and Rhizobium meliloti exopolysaccharide synthesis and their roles in plant interactions;Cangelosi G. A.;J. Bacteriol.,1987
4. Carlson R. W. 1982. Surface chemistry p. 199-234. In W. J. Broughton (ed.) Nitrogen fixation vol. 2. Rhizobium. Clarendon Press Oxford.
5. Carlson R. W. U. R. Bhat and B. Reuhs. 1992. Rhizobium lipopolysaccharides: their structures and evidence for their importance in the nitrogen-fixing symbiotic infection of their host legumes p. 33-44. In P. M. Gresshoff (ed.) Plant biotechnology and development. CRC Press Inc. Boca Raton Fla.
Cited by
144 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献