Affiliation:
1. Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, New Jersey 08544-1014, USA. mdobbelstein@watson.princeton.edu
Abstract
Vaccinia virus contains a gene, termed SPI-2 or B13R, that is closely related in its sequence to a potent inhibitor of apoptosis from cowpox virus (crmA). Infection by vaccinia virus protects HeLa cells against apoptosis that is induced by an immunoglobulin M antibody against the fas receptor or by tumor necrosis factor alpha. This effect is profoundly reduced when the SPI-2 gene is deleted. The SPI-2 gene, when transiently expressed in these cells, can also protect against apoptosis mediated by these agents. Given the similarity to crmA, it seems likely that SPI-2 functions in an analogous fashion, inhibiting the activity of ICE protease family members and blocking the onset of apoptosis.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference37 articles.
1. Ausubel F. M. R. Brent R. E. Kingston D. D. Moore J. G. Seidman J. A. Smith and K. Struhl. 1987. Current protocols in molecular biology vol. 1. John Wiley & Sons Inc. New York.
2. The baculovirus p35 protein inhibits Fas- and tumor necrosis factor-induced apoptosis;Beidler D. R.;J. Biol. Chem.,1995
3. The CTL's kiss of death;Berke G.;Cell,1995
4. A rabbitpox virus serpin gene controls host range by inhibiting apoptosis in restrictive cells;Brooks M. A.;J. Virol.,1995
5. Poxvirus pathogenesis;Buller R. M.;Microbiol. Rev.,1991
Cited by
144 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献