Green Fluorescent Protein-Labeled Monitoring Tool To Quantify Conjugative Plasmid Transfer between Gram-Positive and Gram-Negative Bacteria

Abstract

ABSTRACTOn the basis of pIP501, a green fluorescent protein (GFP)-tagged monitoring tool was constructed for quantifying plasmid mobilization among Gram-positive bacteria and between Gram-positiveEnterococcus faecalisand Gram-negativeEscherichia coli. Furthermore, retromobilization of the GFP-tagged monitoring tool was shown fromE. faecalisOG1X into the clinical isolateE. faecalisT9.