Affiliation:
1. Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada
2. Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada
Abstract
ABSTRACT
Campylobacter
species are important organisms in both human and animal health. The identification of
Campylobacter
currently requires the growth of organisms from complex samples and biochemical identification. In many cases, the condition of the sample being tested and/or the fastidious nature of many
Campylobacter
species has limited the detection of campylobacters in a laboratory setting. To address this, we have designed a set of real-time quantitative PCR (qPCR) assays to detect and quantify 14
Campylobacter
species,
C. coli, C. concisus, C. curvus, C. fetus, C. gracilis, C. helveticus, C. hyointestinalis, C. jejuni, C. lari, C. mucosalis, C. rectus, C. showae, C. sputorum
, and
C. upsaliensis
, directly from DNA extracted from feces. By use of a region of the
cpn60
(also known as
hsp60
or
groEL
) gene, which encodes the universally conserved 60-kDa chaperonin, species-specific assays were designed and validated. These assays were then employed to determine the prevalence of
Campylobacter
species in fecal samples from dogs. Fecal samples were found to contain detectable and quantifiable levels of
C. fetus, C. gracilis, C. helveticus, C. jejuni, C. showae
, and
C. upsaliensis
, with the majority of samples containing multiple
Campylobacter
species. This study represents the first report of
C. fetus, C. gracilis, C. mucosalis
, and
C. showae
detection in dogs and implicates dogs as a reservoir for these species. The qPCR assays described offer investigators a new tool to study many
Campylobacter
species in a culture-independent manner.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
71 articles.
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