Inactivation of polymerase inhibitors for Mycobacterium tuberculosis DNA amplification in sputum by using capture resin-Reference-Cited by-同舟云学术

Inactivation of polymerase inhibitors for Mycobacterium tuberculosis DNA amplification in sputum by using capture resin

Published:1995-03 Issue:3 Volume:33 Page:629-630
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Amicosante M¹,Richeldi L¹,Trenti G¹,Paone G¹,Campa M¹,Bisetti A¹,Saltini C¹

Affiliation:

1. Dipartimento di Scienze Mediche, Oncologiche e Radiologiche, Università di Modena, Italy.

Abstract

Endogenous PCR inhibitors hamper DNA amplification of Mycobacterium tuberculosis DNA in sputum samples. Use of an anion binding resin (GeneReleaser [GR]) improved PCR sensitivity from 77% (27 of 35 culture-positive samples) with phenol-chloroform extraction (P-C) alone to 91% (GR without P-C; P > 0.05) and 100% (P-C and GR combined; P < 0.05).

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.33.3.629-630.1995

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