Host Serine Proteases TMPRSS2 and TMPRSS11D Mediate Proteolytic Activation and Trypsin-Independent Infection in Group A Rotaviruses-Reference-Cited by-同舟云学术

Host Serine Proteases TMPRSS2 and TMPRSS11D Mediate Proteolytic Activation and Trypsin-Independent Infection in Group A Rotaviruses

Published:2021-05-10 Issue:11 Volume:95 Page:
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Sasaki Michihito¹^ORCID,Itakura Yukari¹,Kishimoto Mai¹,Tabata Koshiro¹,Uemura Kentaro¹²³,Ito Naoto⁴,Sugiyama Makoto⁴,Wastika Christida E.¹,Orba Yasuko¹,Sawa Hirofumi¹⁵⁶

Affiliation:

1. Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan

2. Drug Discovery and Disease Research Laboratory, Shionogi & Co., Ltd., Osaka, Japan

3. Laboratory of Biomolecular Science, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan

4. Laboratory of Zoonotic Diseases, Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan

5. Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Sapporo, Japan

6. Global Virus Network, Baltimore, Maryland, USA

Abstract

Proteolytic cleavage of the viral VP4 protein is essential for virion maturation and infectivity in group A rotaviruses (RVAs). In cell culture, RVAs are propagated in culture medium supplemented with the exogenous protease trypsin, which cleaves VP4 and induces the maturation of progeny RVA virions.

Funder

Japan Agency for Medical Research and Development

MEXT | JST | Science and Technology Research Partnership for Sustainable Development

MEXT | Japan Society for the Promotion of Science

Ministry of Education, Culture, Sports, Science and Technology

Uehara Memorial Foundation

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.00398-21