Efficient Multiplication of Human Metapneumovirus in Vero Cells Expressing the Transmembrane Serine Protease TMPRSS2-Reference-Cited by-同舟云学术

Efficient Multiplication of Human Metapneumovirus in Vero Cells Expressing the Transmembrane Serine Protease TMPRSS2

Published:2008-09 Issue:17 Volume:82 Page:8942-8946
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Shirogane Yuta¹,Takeda Makoto¹,Iwasaki Masaharu¹,Ishiguro Nobuhisa²,Takeuchi Hiroki¹,Nakatsu Yuichiro¹,Tahara Maino¹,Kikuta Hideaki³,Yanagi Yusuke¹

Affiliation:

1. Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka 812-8582

2. Department of Pediatrics, Hokkaido University Graduate School of Medicine, Sapporo 060-8638

3. Pediatric Clinic, Touei Hospital, Kita-41, Higashi-6, Higashi-ku, Sapporo 007-0841, Japan

Abstract

ABSTRACT Human metapneumovirus (HMPV) is a major causative agent of severe bronchiolitis and pneumonia. Its fusion (F) protein must be cleaved by host proteases to cause membrane fusion, a critical step for virus infection. By generating Vero cells constitutively expressing the transmembrane serine protease TMPRSS2 and green fluorescent protein-expressing recombinant HMPV, we show that TMPRSS2, which is expressed in the human lung epithelium, cleaves the HMPV F protein efficiently and supports HMPV multiplication. The results indicate that TMPRSS2 is a possible candidate protease involved in the development of lower respiratory tract illness in HMPV-infected patients.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.00676-08

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