Comparative Evaluation of Etest, EUCAST, and CLSI Methods for Amphotericin B, Voriconazole, and Posaconazole against Clinically Relevant Fusarium Species-Reference-Cited by-同舟云学术

Comparative Evaluation of Etest, EUCAST, and CLSI Methods for Amphotericin B, Voriconazole, and Posaconazole against Clinically Relevant Fusarium Species

Published:2017-01 Issue:1 Volume:61 Page:
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Al-Hatmi Abdullah M. S.¹²³,Normand Anne-Cécile⁴,Ranque Stephane⁴^ORCID,Piarroux Renaud⁴,de Hoog G. Sybren¹²,Meletiadis Joseph⁵⁶,Meis Jacques F.⁷⁸^ORCID

Affiliation:

1. CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands

2. Institute of Biodiversity and Ecosystem Dynamics, University of Amsterdam, Amsterdam, The Netherlands

3. Directorate General of Health Services, Ministry of Health, Ibri Hospital, Ibri, Oman

4. Aix-Marseille University, APHM, CHU Timone, IP-TPT, Parasitology-Mycology, Marseille, France

5. Clinical Microbiology Laboratory, Attikon University Hospital, Medical School, National and Kapodistrian University of Athens, Athens, Greece

6. Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands

7. Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands

8. Department of Medical Microbiology, Radboud University Medical Centre, Nijmegen, The Netherlands

Abstract

ABSTRACT We compared EUCAST and CLSI methods versus Etest for antifungal susceptibility testing of 20 clinically relevant Fusarium species against amphotericin B, posaconazole, and voriconazole. The median Etest amphotericin B and posaconazole MICs were 1 dilution higher than the median EUCAST and the CLSI MICs. The essential agreement (within ±1/±2 dilutions) was 60/90%, 80/95%, and 70/85% between the Etest and EUCAST methods and 80/95%, 75/95%, and 45/100% between the Etest and CLSI methods for amphotericin B, voriconazole, and posaconazole, respectively. The categorical agreement was >85%. Etest can be used for antifungal susceptibility testing of Fusarium species.

Funder

Ministry of Health

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.01671-16

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