Rapid identification of clinical members of Fusarium fujikuroi complex using MALDI-TOF MS-Reference-Cited by-同舟云学术

Rapid identification of clinical members of Fusarium fujikuroi complex using MALDI-TOF MS

Published:2015-12 Issue:12 Volume:10 Page:1939-1952
ISSN:1746-0913
Container-title:Future Microbiology
language:en
Short-container-title:Future Microbiology

Author:

Al-Hatmi Abdullah MS¹²³,Normand Anne-Cécile⁴,van Diepeningen Anne D¹,Hendrickx Marijke⁵,de Hoog G Sybren¹²⁶⁷⁸,Piarroux Renaud⁴

Affiliation:

1. CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands

2. Institute of Biodiversity & Ecosystem Dynamics, University of Amsterdam, Amsterdam, The Netherlands

3. Directorate General of Health Services, Ministry of Health, Ibri Hospital, Ibri, Oman

4. Laboratoire de Parasitologie-Mycologie, CHU Timone, Université de la Méditerranée, Marseille, France

5. BCCM/IHEM: Scientific Institute of Public Health, Mycology & Aerobiology Section, Bruxelles, Belgium

6. Peking University Health Science Center, Research Center for Medical Mycology, Beijing, China

7. Shanghai Institute of Medical Mycology, Changzheng Hospital, Second Military Medical University, Shanghai, China

8. King Abdulaziz University, Jeddah, Saudi Arabia

Abstract

Aim: To develop the matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF MS) method for identification of Fusarium species within Fusarium fujikuroi complex for use in clinical microbiology laboratories. Materials & methods: A total of 24 reference and 60 clinical and environmental isolates belonging to the F. fujikuroi complex were subjected to MALDI-TOF MS identification. Protein extracts of Fusarium isolates were obtained using formic acid extraction. Multilocus sequence analysis was used as a gold standard. Results: The MALDI-TOF MS Biotyper correctly identified 93.6% of the strains down to the species level, while the remaining isolates (6.4%) were identified at the genus level when using scores of ≥ 2.0 as cut-off values. Correct identification was obtained despite large intraspecific heterogeneities in MALDI-TOF spectra. Conclusion: MALDI-TOF MS is a rapid identification tool for the recognition of species within F. fujikuroi complex, provided a database is available.

Publisher

Future Medicine Ltd

Subject

Microbiology (medical),Microbiology

Link

https://www.futuremedicine.com/doi/pdf/10.2217/fmb.15.108

Reference42 articles.

1. Molecular Phylogenetic Diversity of Dermatologic and Other Human Pathogenic Fusarial Isolates from Hospitals in Northern and Central Italy

2. Improvement in the outcome of invasive fusariosis in the last decade

3. InvasiveFusariuminfections: a retrospective survey of 31 cases

4. Use of the DiversiLab System for Species and Strain Differentiation of Fusarium Species Isolates

5. Fusarium Infections in Immunocompromised Patients

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