A Practical Workflow for the Identification of Aspergillus, Fusarium, Mucorales by MALDI-TOF MS: Database, Medium, and Incubation Optimization

Author:

Barker Kevin R.123ORCID,Kus Julianne V.24ORCID,Normand Anne-Cecile5ORCID,Gharabaghi Farhad6,McTaggart Lisa4,Rotstein Coleman7,Richardson Susan E.26,Campigotto Aaron26,Tadros Manal26

Affiliation:

1. Division of Microbiology, Department of Laboratory Medicine and Genetics, Trillium Health Partners, Mississauga, Ontario, Canada

2. Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada

3. Institute for Better Health, Trillium Health Partners, Mississauga, Ontario, Canada

4. Public Health Ontario, Toronto, Ontario, Canada

5. Service de Parasitologie Mycologie, AP-HP, Groupe Hospitalier La Pitié-Salpêtrière, Paris, France

6. Division of Microbiology, Department of Paediatric Laboratory Medicine, Hospital for Sick Children, Toronto, Ontario, Canada

7. Division of Infectious Diseases, Department of Medicine, Immunocompromised Host Infectious Diseases Service, University Health Network, Toronto, Canada

Abstract

There is an increasing body of literature on the utility of MALDI-TOF MS in the identification of filamentous fungi. However, the process still lacks standardization.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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