Quantitative Microbial Risk Assessment of Pathogenic Vibrios in Marine Recreational Waters of Southern California

Abstract

ABSTRACTThis study investigated the occurrence of three types of vibrios in Southern California recreational beach waters during the peak marine bathing season in 2007. Over 160 water samples were concentrated and enriched for the detection of vibrios. Four sets of PCR primers, specific forVibrio cholerae,V. parahaemolyticus, andV. vulnificusspecies and theV. parahaemolyticustoxin gene, respectively, were used for the amplification of bacterial genomic DNA. Of 66 samples from Doheny State Beach, CA, 40.1% were positive forV. choleraeand 27.3% were positive forV. parahaemolyticus, and 1 sample (1.5%) was positive for theV. parahaemolyticustoxin gene. Of the 96 samples from Avalon Harbor, CA, 18.7% were positive forV. cholerae, 69.8% were positive forV. parahaemolyticus, and 5.2% were positive for theV. parahaemolyticustoxin gene. The detection of theV. choleraegenetic marker was significantly more frequent at Doheny State Beach, while the detection of theV. parahaemolyticusgenetic marker was significantly more frequent at Avalon Harbor. A probability-of-illness model forV. parahaemolyticuswas applied to the data. The risk for bathers exposed to recreational waters at two beaches was evaluated through Monte Carlo simulation techniques. The results suggest that the microbial risk from vibrios during beach recreation was below the illness benchmark set by the U.S. EPA. However, the risk varied with location and the type of water recreation activities. Surfers and children were exposed to a higher risk of vibrio diseases. Microbial risk assessment can serve as a useful tool for the management of risk related to opportunistic marine pathogens.