Enhanced Selenate Accumulation in Cupriavidus metallidurans CH34 Does Not Trigger a Detoxification Pathway

Author:

Avoscan Laure1,Carrière Marie1,Proux Olivier2,Sarret Géraldine3,Degrouard Jéril4,Covès Jacques5,Gouget Barbara1

Affiliation:

1. Laboratoire Pierre Süe, Groupe Toxicologie Humaine et Environnementale, CEA-CNRS UMR 9956, F91191 Gif-sur-Yvette, France

2. CRG-FAME Beamline, European Synchrotron Radiation Facility, F-38043 Grenoble, France

3. Environmental Geochemistry Group, LGIT, University of Grenoble and CNRS, BP 53, 38041 Grenoble Cedex 9, France

4. Laboratoire Développement, Morphogenèse et Evolution, Université Paris 11-CNRS UMR 8080, F91405 Orsay, France

5. Laboratoire des Protéines Membranaires Institut de Biologie Structurale—Jean-Pierre Ebel, UMR 5075 CNRS-CEA-UJF, 41 Rue Jules Horowitz, 38027 Grenoble Cedex, France

Abstract

ABSTRACT Cupriavidus metallidurans CH34 cells grown under sulfate-limited conditions accumulated up to six times more selenate than cells grown in sulfate-rich medium. The products of selenate reduction detected by X-ray absorption spectroscopy, electron microscopy, and energy-dispersive X-ray analysis did not define this strain as being a good candidate for bioremediation of selenate-contaminated environments.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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