Affiliation:
1. Department of Pathology and Program in Infectious Diseases, University of Texas Medical School, M.D. Anderson Hospital and Tumor Institute, Houston, Texas 77025
Abstract
A radioimmunoassay for tobramycin was developed using antisera from rabbits injected with a tobramycin-bovine serum albumin conjugate. The antibody had a relatively high affinity for
125
I-labeled tobramycin (1.6 × 10
9
liters/mol), thus indicating little interference in immunoreactivity by iodination. The radioimmunoassay was highly specific, and only minor cross-reaction occurred with other aminoglycoside antibiotics. The radioimmunoassay was also more sensitive than previously published tobramycin assays (minimum sensitivity, 280 pg), and there was a significant correlation (
P
< 0.05) with both the radioenzymatic and microbiological assays.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
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