Affiliation:
1. Department of Pathology, The University of Texas Medical School, Houston, Texas 77025
Abstract
An antibody induced in rabbits against a gentamicin-bovine serum albumin conjugate cross-reacted with the chemically related aminoglycoside, sisomicin, facilitating the development of a sisomicin radioimmunoassay. Sisomicin was labeled with
125
I using an acylating agent [3-(4-hydroxyphenyl)propionic acid-
N
-hydroxy-succinimide ester]. The resulting assay produced a linear standard curve on a logit-log plot with a sensitivity of 140 pg. Comparison with a microbiological assay showed no significant difference (
P
< 0.001) in the measurement of sisomicin by these two methods.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
Cited by
14 articles.
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