Genetic Analysis, Using P22 Challenge Phage, of the Nitrogen Activator Protein DNA-Binding Site in the Klebsiella aerogenes put Operon-Reference-Cited by-同舟云学术

Genetic Analysis, Using P22 Challenge Phage, of the Nitrogen Activator Protein DNA-Binding Site in the Klebsiella aerogenes put Operon

Published:1998-02 Issue:3 Volume:180 Page:571-577
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Chen Li-Mei¹,Goss Thomas J.²,Bender Robert A.²,Swift Simon³,Maloy Stanley¹

Affiliation:

1. Department of Microbiology, University of Illinois, Urbana, Illinois 618011

2. Department of Biology, University of Michigan, Ann Arbor, Michigan 48109-10482;

3. Department of Applied Biochemistry and Food Science, University of Nottingham, Sutton Bonington Campus, Leicestershire LE12 5RD, United Kingdom3; and

Abstract

ABSTRACT The nac gene product is a LysR regulatory protein required for nitrogen regulation of several operons from Klebsiella aerogenes and Escherichia coli . We used P22 challenge phage carrying the put control region from K. aerogenes to identify the nucleotide residues important for nitrogen assimilation control protein (NAC) binding in vivo. Mutations in an asymmetric 30-bp region prevented DNA binding by NAC. Gel retardation experiments confirmed that NAC specifically binds to this sequence in vitro, but NAC does not bind to the corresponding region from the put operon of Salmonella typhimurium , which is not regulated by NAC.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.180.3.571-577.1998

Reference28 articles.

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3. Cloning of the Klebsiella aerogenes nac gene, which encodes a factor required for nitrogen regulation of the histidine utilization (hut) operons in Salmonella typhimurium

4. Regulation of proline utilization in enteric bacteria: cloning and characterization of the Klebsiella put control region

5. Tn5tac1, a derivative of transposon Tn5 that generates conditional mutants;Chow W.;Proc. Natl. Acad. Sci. USA,1988

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