Substitutions at Auxiliary Operator O3 Enhance Repression by Nitrate-Responsive Regulator NarL at Synthetic lac Control Regions in Escherichia coli K-12-Reference-Cited by-同舟云学术

Substitutions at Auxiliary Operator O3 Enhance Repression by Nitrate-Responsive Regulator NarL at Synthetic lac Control Regions in Escherichia coli K-12

Published:2008-01 Issue:1 Volume:190 Page:428-433
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Stewart Valley¹,Bledsoe Peggy J.¹

Affiliation:

1. Section of Microbiology, University of California, Davis, California 95616-8665

Abstract

ABSTRACT We constructed monocopy lac operon control regions in which the operators O1- lac and O3- lac were replaced by NarL and NarP binding sites from the nirB or napF operon control regions. The results support the hypothesis that DNA-bound dimers of phospho-NarL can participate in higher-order cooperative interactions.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.01431-07

Reference47 articles.

1. Alberti, S., S. Oehler, B. von Wilcken-Bergmann, H. Krämer, and B. Müller-Hill. 1991. Dimer-to-tetramer assembly of Lac repressor involves a leucine heptad repeat. New Biol. 3 : 57-62.

2. Alberti, S., S. Oehler, B. von Wilcken-Bergmann, and B. Müller-Hill. 1993. Genetic analysis of the leucine heptad repeats of Lac repressor: evidence for a 4-helical bundle. EMBO J. 12 : 3227-3236.

3. Becker, N. A., J. D. Kahn, and L. J. Maher III. 2005. Bacterial repression loops require enhanced DNA flexibility. J. Mol. Biol. 349 : 716-730.

4. Bock, A., M. Bantscheff, A. L. Perraud, K. Rippe, V. Weiss, M. O. Glocker, and R. Gross. 2001. Rational design and molecular characterization of a chimaeric response regulator protein. J. Mol. Biol. 310 : 283-290.

5. Towards Single-Copy Gene Expression Systems Making Gene Cloning Physiologically Relevant: Lambda InCh, a Simple Escherichia coli Plasmid-Chromosome Shuttle System

Cited by 1 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Functional roles for the GerE-family carboxyl-terminal domains of nitrate response regulators NarL and NarP of Escherichia coli K-12;Microbiology;2010-10-01