Affiliation:
1. Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115
Abstract
ABSTRACT
We describe a simple system for reversible, stable integration of plasmid-borne genes into the
Escherichia coli
chromosome. Most ordinary
E. coli
strains and a variety of pBR322-derived ampicillin-resistant plasmids can be used. A single genetic element, a lambda phage, is the only specialized vector required. The resultant strains have a single copy of the plasmid fragment inserted stably at the lambda attachment site on the chromosome, with nearly the entire lambda genome deleted.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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