Extraction-Free Methods for the Detection of SARS-CoV-2 by Reverse Transcription-PCR: a Comparison with the Cepheid Xpert Xpress SARS-CoV-2 Assay across Two Medical Centers-Reference-Cited by-同舟云学术

Extraction-Free Methods for the Detection of SARS-CoV-2 by Reverse Transcription-PCR: a Comparison with the Cepheid Xpert Xpress SARS-CoV-2 Assay across Two Medical Centers

Published:2021-01-21 Issue:2 Volume:59 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Cameron Andrew¹,Pecora Nicole D.¹²^ORCID,Pettengill Matthew A.³^ORCID

Affiliation:

1. University of Rochester Medical Center, Clinical Microbiology, Department of Pathology and Laboratory Medicine, Rochester, New York, USA

2. University of Rochester Medical Center, Department of Microbiology and Immunology, Rochester, New York, USA

3. Thomas Jefferson University Hospital, Department of Pathology, Anatomy, and Cell Biology, Philadelphia, Pennsylvania, USA

Funder

Departmental funds

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.02643-20

Reference6 articles.

1. Use of a simplified sample processing step without RNA extraction for direct SARS-CoV-2 RT-PCR detection

2. Detection of SARS-CoV-2 RNA by direct RT-qPCR on nasopharyngeal specimens without extraction of viral RNA

3. SARS-CoV-2 detection by direct rRT-PCR without RNA extraction

4. Direct RT-qPCR detection of SARS-CoV-2 RNA from patient nasopharyngeal swabs without an RNA extraction step

5. Saliva or Nasopharyngeal Swab Specimens for Detection of SARS-CoV-2

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4. Ultrafast RNA extraction-free SARS-CoV-2 detection by direct RT-PCR using a rapid thermal cycling approach;Diagnostic Microbiology and Infectious Disease;2023-09

5. Optimization of Extraction-Free Protocols for SARS-CoV-2 Detection using a Commercial rRT-PCR Assay;2023-08-29