High-Throughput COVID-19 Testing of Naso-Oropharyngeal Swabs Using a Sensitive Extraction-Free Sample Preparation Method-Reference-Cited by-同舟云学术

High-Throughput COVID-19 Testing of Naso-Oropharyngeal Swabs Using a Sensitive Extraction-Free Sample Preparation Method

Published:2022-08-31 Issue:4 Volume:10 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Pryce Todd M.¹^ORCID,Haygarth Erin J.¹,Bordessa Jessica¹,Jeffery Courtney T.¹,Kay Ian D.¹,Flexman James P.¹,Boan Peter A.¹²^ORCID

Affiliation:

1. PathWest Laboratory Medicine WA, Department of Clinical Microbiology, Fiona Stanley Hospital, Murdoch, Western Australia, Australia

2. Department of Infectious Diseases, Fiona Stanley Fremantle Hospital Group, Murdoch, Western Australia, Australia

Abstract

Laboratories have experienced extraordinary demand globally for reagents, consumables, and instrumentation, while facing unprecedented testing demand needed for the diagnosis of SARS-CoV-2 infection. A major bottleneck in testing throughput is the purification of viral RNA.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.01358-22

Reference26 articles.

1. Diagnostic strategies for SARS-CoV-2 infection and interpretation of microbiological results

2. Centers for Disease Control and Prevention. 2020. Implementation of mitigation strategies for communities with local Covid-19 transmission. https://www.cdc.gov/coronavirus/2019-ncov/community/community-mitigation.html.

3. Thermal treatment of nasopharyngeal samples before cobas SARS-CoV-2 testing

4. Qualitative and quantitative effects of thermal treatment of naso-oropharyngeal samples before cobas SARS-CoV-2 testing

5. Evaluation of a PlexZyme-Based PCR Assay and Assessment of COVID-19 Surge Testing Throughput Compared to Cobas SARS-CoV-2