Affiliation:
1. Department of Bioresources, ARC Seibersdorf Research GmbH, Seibersdorf
2. Institute for Forest Ecology, Federal Office and Research Centre for Forests, Vienna, Austria
Abstract
ABSTRACT
The diversity and composition of soil bacterial communities were compared among six Austrian natural forests, including oak-hornbeam, spruce-fir-beech, and Austrian pine forests, using terminal restriction fragment length polymorphism (T-RFLP, or TRF) analysis and sequence analysis of 16S rRNA genes. The forests studied differ greatly in soil chemical characteristics, microbial biomass, and nutrient turnover rates. The aim of this study was to relate these differences to the composition of the bacterial communities inhabiting the individual forest soils. Both TRF profiling and clone sequence analysis revealed that the bacterial communities in soils under Austrian pine forests, representing azonal forest types, were distinct from those in soils under zonal oak-hornbeam and spruce-fir-beech forests, which were more similar in community composition. Clones derived from an Austrian pine forest soil were mostly affiliated with high-G+C gram-positive bacteria (49%), followed by members of the α
-Proteobacteria
(20%) and the
Holophaga/Acidobacterium
group (12%). Clones in libraries from oak-hornbeam and spruce-fir-beech forest soils were mainly related to the
Holophaga/Acidobacterium
group (28 and 35%), followed by members of the
Verrucomicrobia
(24%) and the α
-Proteobacteria
(27%), respectively. The soil bacterial communities in forests with distinct vegetational and soil chemical properties appeared to be well differentiated based on 16S rRNA gene phylogeny. In particular, the outstanding position of the Austrian pine forests, which are determined by specific soil conditions, was reflected in the bacterial community composition.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
163 articles.
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