3-Nitroadipate, a Metabolic Intermediate for Mineralization of 2,4-Dinitrophenol by a New Strain of a Rhodococcus Species

Author:

Blasco Rafael1,Moore Edward2,Wray Victor2,Pieper Dietmar2,Timmis Kenneth2,Castillo Francisco1

Affiliation:

1. Departamento de Bioquı́mica y Biologı́a Molecular, Facultad de Ciencias, 14071 Córdoba, Spain,1 and

2. GBF (National Research Center for Biotechnology), D-38124 Braunschweig, Germany2

Abstract

ABSTRACT The bacterial strain RB1 has been isolated by enrichment cultivation with 2,4-dinitrophenol as the sole nitrogen, carbon, and energy source and characterized, on the basis of 16S rRNA gene sequence comparison, as a Rhodococcus species closely related to Rhodococcus opacus. Rhodococcus sp. strain RB1 degrades 2,4-dinitrophenol, releasing the two nitro groups from the compound as nitrite. The release of nitro groups from 2,4-dinitrophenol occurs in two steps. First, the 2-nitro group is removed as nitrite, with the production of an aliphatic nitro compound identified by 1 H nuclear magnetic resonance and mass spectrometry as 3-nitroadipate. Then, this metabolic derivative is further metabolized, releasing its nitro group as nitrite. Full nitrite assimilation upon reduction to ammonia requires that an additional carbon source be supplied to the medium.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

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