Affiliation:
1. Departamento de Bioquı́mica y Biologı́a Molecular, Facultad de Ciencias, 14071 Córdoba, Spain,1 and
2. GBF (National Research Center for Biotechnology), D-38124 Braunschweig, Germany2
Abstract
ABSTRACT
The bacterial strain RB1 has been isolated by enrichment cultivation with 2,4-dinitrophenol as the sole nitrogen, carbon, and energy source and characterized, on the basis of 16S rRNA gene sequence comparison, as a
Rhodococcus
species closely related to
Rhodococcus opacus. Rhodococcus
sp. strain RB1 degrades 2,4-dinitrophenol, releasing the two nitro groups from the compound as nitrite. The release of nitro groups from 2,4-dinitrophenol occurs in two steps. First, the 2-nitro group is removed as nitrite, with the production of an aliphatic nitro compound identified by
1
H nuclear magnetic resonance and mass spectrometry as 3-nitroadipate. Then, this metabolic derivative is further metabolized, releasing its nitro group as nitrite. Full nitrite assimilation upon reduction to ammonia requires that an additional carbon source be supplied to the medium.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
49 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献