Use of an Escherichia coli Recombinant Producing Thermostable Polyphosphate Kinase as an ATP Regenerator To Produce Fructose 1,6-Diphosphate-Reference-Cited by-同舟云学术

Use of an Escherichia coli Recombinant Producing Thermostable Polyphosphate Kinase as an ATP Regenerator To Produce Fructose 1,6-Diphosphate

Published:2007-09 Issue:17 Volume:73 Page:5676-5678
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Iwamoto Seishi¹,Motomura Kei¹,Shinoda Yasuharu¹,Urata Masaaki¹,Kato Junichi¹,Takiguchi Noboru¹,Ohtake Hisao²,Hirota Ryuichi¹,Kuroda Akio¹

Affiliation:

1. Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8530, Japan

2. Department of Biotechnology, Osaka University, Suita, Osaka 565-0871, Japan

Abstract

ABSTRACT Heat-treated Escherichia coli producing Thermus polyphosphate kinase regenerated ATP by using exogenous polyphosphate. This recombinant could be used as a platform to produce valuable compounds in combination with thermostable phosphorylating or energy-requiring enzymes. In this work, we demonstrated the production of fructose 1,6-diphosphate from fructose and polyphosphate.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.00278-07

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