Affiliation:
1. College of Light Industry and Food Engineering Guangxi University Nanning China
2. Guangxi South Subtropical Agricultural Sciences Research Institute Longzhou China
Abstract
AbstractBACKGROUNDAs a rare hexose with low calories and various physiological functions, d‐allulose has drawn increasing attention. The current industrial production of d‐allulose from d‐fructose or d‐glucose is achieved via epimerization based on the Izumoring strategy; however, the inherent reaction equilibrium during reversible reaction limits its high conversion yield. Although the conversion of d‐fructose to d‐allulose could be enhanced via phosphorylation‐dephosphorylation mediated by metabolic engineering, biomass reduction and byproduct accumulation remain a largely unresolved issue.RESULTSAfter modifying the glycolytic pathway of Escherichia coli and optimizing the whole‐cell reaction condition, the engineered strain produced 7.57 ± 0.61 g L−1 d‐allulose from 30 g L−1 d‐glucose after 24 h of catalysis. By developing an ATP regeneration system for enhanced substrate phosphorylation, the cell growth inhibition was alleviated and d‐allulose production increased by 55.3% to 11.76 ± 0.58 g L−1 (0.53 g g−1). Fine‐tuning of carbon flux caused a 48% reduction in d‐fructose accumulation to 1.47 ± 0.15 g L−1. After implementing fed‐batch co‐substrate strategy, the d‐allulose titer reached 15.80 ± 0.31 g L−1 (0.62 g g−1) with a d‐glucose conversion rate of 84.8%.CONCLUSIONThe present study reports a novel strategy for high‐yield d‐allulose production from low‐cost substrate. © 2023 Society of Chemical Industry.
Funder
National Natural Science Foundation of China
Natural Science Foundation of Guangxi Province
Subject
Nutrition and Dietetics,Agronomy and Crop Science,Food Science,Biotechnology
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献