Real-Time PCR Assay Targets the 23S-5S Spacer for Direct Detection and Differentiation of Legionella spp. and Legionella pneumophila-Reference-Cited by-同舟云学术

Real-Time PCR Assay Targets the 23S-5S Spacer for Direct Detection and Differentiation of Legionella spp. and Legionella pneumophila

Published:2003-10 Issue:10 Volume:41 Page:4815-4816
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Herpers Bjorn L.¹,de Jongh Bartelt M.¹,van der Zwaluw Kim²,van Hannen Erik J.¹

Affiliation:

1. Department of Medical Microbiology and Immunology, St. Antonius Hospital, Koekoekslaan 1, 3435 CM Nieuwegein

2. Diagnostic Laboratory for Infectious Diseases and Perinatal Screening (LIS), National Institute of Public Health and the Environment (RIVM), 3720 BA Bilthoven, The Netherlands

Abstract

ABSTRACT A real-time PCR for the ABI Prism 7000 system targeting the 23S-5S spacer of Legionella spp. was developed. Simultaneous detection and differentiation of Legionella spp. and Legionella pneumophila within 90 min and without post-PCR melting-curve analysis was achieved using two TaqMan probes. In sputum samples from 23 controls and 17 patients with legionellosis, defined by positive culture, urinary antigen testing, or seroconversion, 94% sensitivity and 100% specificity were observed.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.41.10.4815-4816.2003

Reference16 articles.

1. Detection of Legionella pneumophila Using a Real-Time PCR Hybridization Assay

2. A Legionella pneumophila gene encoding a species-specific surface protein potentiates initiation of intracellular infection

3. Cianciotto, N. P., W. O'Connell, G. A. Dasch, and L. P. Mallavia. 1995. Detection of mip-like sequences and Mip-related proteins within the family Rickettsiaceae. Curr. Microbiol.30:149-153.

4. Detection of Legionella Species in Respiratory Specimens Using PCR with Sequencing Confirmation

5. De Rijk, P., and R. De Wachter. 1993. DCSE, an interactive tool for sequence alignment and secondary structure research. Comput. Appl. Biosci.9:735-740.

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