Affiliation:
1. Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Chuo-ku, Chiba 260-8673, Japan
Abstract
ABSTRACT
Mitochondrial cytochrome
b
genes (
cyt b
) of 40 strains of
Cryptococcus neoformans
were partially sequenced to determine the genetic relations. With the exception of the type strain of
C. neoformans
var.
neoformans
, all strains contained introns in their sequences. Analysis of 386 bp of coding sequence from each strain under investigation revealed a total of 27 (6.99%) variable nucleotide sites and categorized isolates of
C. neoformans
into nine
cyt b
types.
C. neoformans
var.
gattii
included
cyt b
types I to V, and
C. neoformans
var.
neoformans
comprised types VI to IX.
cyt b
types were correlated with serotypes. All strains with
cyt b
types I, IV, and V were serotype B. All other strains except IFM 5878 (serotype B) with
cyt b
types II and III were serotype C. Serotype D strains had
cyt b
types VI and IX, and serotype A strains were
cyt b
type VIII. Of four serotype AD strains, one was
cyt b
type VII and the remaining three were type VIII. The phylogenetic tree based on deduced amino acid sequences divided the strains only into
C. neoformans
var.
neoformans
and
C. neoformans
var.
gattii
. These results indicate that
cyt b
sequences are effective for DNA typing as well as phylogenetic analysis of
C. neoformans
.
Publisher
American Society for Microbiology
Cited by
24 articles.
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