Iterative Combinatorial Mutagenesis as an Effective Strategy for Generation of Deacetoxycephalosporin C Synthase with Improved Activity toward Penicillin G-Reference-Cited by-同舟云学术

Iterative Combinatorial Mutagenesis as an Effective Strategy for Generation of Deacetoxycephalosporin C Synthase with Improved Activity toward Penicillin G

Published:2012-08-24 Issue:21 Volume:78 Page:7809-7812
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl. Environ. Microbiol.

Author:

Ji Junjie,Fan Keqiang,Tian Xiuyun,Zhang Xia,Zhang Yuxiu,Yang Keqian

Abstract

ABSTRACTAn iterative combinatorial mutagenesis (ICM) strategy was used to engineer deacetoxycephalosporin C synthase ofStreptomyces clavuligerus(scDAOCS) for improved activity toward penicillin G. Seven mutational sites were repeatedly combined onto a starter mutant (C155Y Y184H V275I C281Y) of scDAOCS. Eleven improved combinatorial mutants were identified from 24 mutants in four rounds of ICM.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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