Monoclonal antibodies to Nocardia asteroides and Nocardia brasiliensis antigens-Reference-Cited by-同舟云学术

Monoclonal antibodies to Nocardia asteroides and Nocardia brasiliensis antigens

Published:1990-01 Issue:1 Volume:28 Page:87-91
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Jiménez T¹,Díaz A M¹,Zlotnik H¹

Affiliation:

1. Department of Microbiology and Medical Zoology, School of Medicine, University of Puerto Rico, San Juan 00936.

Abstract

Nocardia asteroides and Nocardia brasiliensis whole-cell extracts were used as antigens to generate monoclonal antibodies (MAbs). Six stable hybrid cell lines secreting anti-Nocardia spp. MAbs were obtained. These were characterized by enzyme-linked immunosorbent assay, Western blot (immunoblot), and immunofluorescence assay. Although all the MAbs exhibited different degrees of cross-reactivity with N. asteroides and N. brasiliensis antigens as well as with culture-filtrate antigens from Mycobacteria spp., they have the potential for use as reagents in the purification of Nocardia antigens.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.28.1.87-91.1990

Reference17 articles.

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